Detail Information of Post-Translational Modifications

General Information of Drug Transporter (DT)
DT ID	DTD0462 Transporter Info
Gene Name	SLC7A1
Transporter Name	High affinity cationic amino acid transporter 1
Gene ID	6541
UniProt ID	P30825

Post-Translational Modification of This DT
Overview ofSLC7A1 Modification Sites with Functional and Structural Information
Sequence	M G C K V L L N I G Q Q M L R R K V V D C S R E E T R L S R C L N T F D L V A L G V G S T L G A G V Y V L A G A V A R E N A G P A I V I S F L I A A L A S V L A G L C Y G E F G A R V P K T G S A Y L Y S Y V T V G E L W A F I T G W N L I L S Y I I G T S S V A R A W S A T F D E L I G R P I G E F S R T H M T L N A P G V L A E N P D I F A V I I I L I L T G L L T L G V K E S A M V N K I F T C I N V L V L G F I M V S G F V K G S V K N W Q L T E E D F G N T S G R L C L N N D T K E G K P G V G G F M P F G F S G V L S G A A T C F Y A F V G F D C I A T T G E E V K N P Q K A I P V G I V A S L L I C F I A Y F G V S A A L T L M M P Y F C L D N N S P L P D A F K H V G W E G A K Y A V A V G S L C A L S A S L L G S M F P M P R V I Y A M A E D G L L F K F L A N V N D R T K T P I I A T L A S G A V A A V M A F L F D L K D L V D L M S I G T L L A Y S L V A A C V L V L R Y Q P E Q P N L V Y Q M A S T S D E L D P A D Q N E L A S T N D S Q L G F L P E A E M F S L K T I L S P K N M E P S K I S G L I V N I S T S L I A V L I I T F C I V T V L G R E A L T K G A L W A V F L L A G S A L L C A V V T G V I W R Q P E S K T K L S F K V P F L P V L P I L S I F V N V Y L M M Q L D Q G T W V R F A V W M L I G F I I Y F G Y G L W H S E E A S L D A D Q A R T P D G N L D Q C K
PTM type	X-S-nitrosylation X-S-palmitoylation X-Ubiquitination X-Ubiquitination X: Amino Acid

S-nitrosylation
Cystine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC7A1			[1]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	21
Experimental Method	Co-Immunoprecipitation
Detailed Description	S-nitrosylation (-SNO) at SLC7A1 Cystine 21 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC7A1			[1], [2]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	628
Experimental Method	Co-Immunoprecipitation
Detailed Description	S-nitrosylation (-SNO) at SLC7A1 Cystine 628 has the potential to affect its expression or activity.
S-palmitoylation
Cystine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC7A1			[3], [4]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	3
Experimental Method	Co-Immunoprecipitation
Detailed Description	S-palmitoylation at SLC7A1 Cystine 3 has the potential to affect its expression or activity.
Ubiquitination
Lysine	6 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	.			[5]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	4
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC7A1 Lysine 4 has the potential to affect its expression or activity.
PTM Phenomenon2	.			[5]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	373
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC7A1 Lysine 373 has the potential to affect its expression or activity.
PTM Phenomenon3	.			[5]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	373
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC7A1 Lysine 373 has the potential to affect its expression or activity.
PTM Phenomenon4	.			[5]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	484
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC7A1 Lysine 484 has the potential to affect its expression or activity.
PTM Phenomenon5	.			[5]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	484
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC7A1 Lysine 484 has the potential to affect its expression or activity.
PTM Phenomenon6	.			[5]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	484
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC7A1 Lysine 484 has the potential to affect its expression or activity.
Unclear Residue	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Decreasing cell surface expression of SLC7A1			[6]
Role of PTM	Surface Expression Modulation
Related Enzyme	E3 ubiquitin-protein ligase NEDD4 (NEDD4) E3 ubiquitin-protein ligase NEDD4-like (NEDD4L)
Experimental Material(s)	Human embryonic kidney 293 (HEK293) cells
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC7A1 have been reported to decrease its cell surface expression.

References
1	Proteome-wide detection of S-nitrosylation targets and motifs using bioorthogonal cleavable-linker-based enrichment and switch technique. Nat Commun. 2019 May 16;10(1):2195.
2	Dual Labeling Biotin Switch Assay to Reduce Bias Derived From Different Cysteine Subpopulations: A Method to Maximize S-Nitrosylation Detection. Circ Res. 2015 Oct 23;117(10):846-57.
3	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites. J Proteome Res. 2018 May 4;17(5):1907-1922.
4	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes. Mol Cell Proteomics. 2010 Jan;9(1):54-70.
5	ActiveDriverDB: human disease mutations and genome variation in post-translational modification sites of proteins. Nucleic Acids Res. 2018;46(D1):D901-D910.
6	Protein kinase C-dependent ubiquitination and clathrin-mediated endocytosis of the cationic amino acid transporter CAT-1. J Biol Chem. 2011 Mar 11;286(10):8697-8706.

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Detail Information of Post-Translational Modifications

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