Detail Information of Post-Translational Modifications

General Information of Drug Transporter (DT)
DT ID	DTD0251 Transporter Info
Gene Name	SLC2A1
Transporter Name	Glucose transporter type 1, erythrocyte/brain
Gene ID	6513
UniProt ID	P11166

Post-Translational Modification of This DT
Overview ofSLC2A1 Modification Sites with Functional and Structural Information
Sequence	M E P S S K K L T G R L M L A V G G A V L G S L Q F G Y N T G V I N A P Q K V I E E F Y N Q T W V H R Y G E S I L P T T L T T L W S L S V A I F S V G G M I G S F S V G L F V N R F G R R N S M L M M N L L A F V S A V L M G F S K L G K S F E M L I L G R F I I G V Y C G L T T G F V P M Y V G E V S P T A L R G A L G T L H Q L G I V V G I L I A Q V F G L D S I M G N K D L W P L L L S I I F I P A L L Q C I V L P F C P E S P R F L L I N R N E E N R A K S V L K K L R G T A D V T H D L Q E M K E E S R Q M M R E K K V T I L E L F R S P A Y R Q P I L I A V V L Q L S Q Q L S G I N A V F Y Y S T S I F E K A G V Q Q P V Y A T I G S G I V N T A F T V V S L F V V E R A G R R T L H L I G L A G M A G C A I L M T I A L A L L E Q L P W M S Y L S I V A I F G F V A F F E V G P G P I P W F I V A E L F S Q G P R P A A I A V A G F S N W T S N F I V G M C F Q Y V E Q L C G P Y V F I I F T V L L V L F F I F T Y F K V P E T K G R T F D E I A S G F R Q G G A S Q S D K T P E E L F H P L G A D S Q V
PTM type	X-Acetylation X-Deubiquitination X-N-glycosylation X-N-linked glycosylation X-O-glycosylation X-O-linked glycosylation X-Phosphorylation X-Phosphorylation X-S-palmitoylation X-Sulfoxidation X-SUMOylation X-Ubiquitination X-Ubiquitination X: Amino Acid

Acetylation
Lysine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC2A1			[1]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	245
Experimental Method	Co-Immunoprecipitation
Detailed Description	Acetylation at SLC2A1 Lysine 245 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC2A1			[1]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	477
Experimental Method	Co-Immunoprecipitation
Detailed Description	Acetylation at SLC2A1 Lysine 477 has the potential to affect its expression or activity.
Methionine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC2A1			[2]
Role of PTM	Potential impacts
Modified Residue	Methionine	Modified Location	1
Experimental Method	Co-Immunoprecipitation
Detailed Description	Acetylation at SLC2A1 Methionine 1 has the potential to affect its expression or activity.
Deubiquitination
Unclear Residue	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Increasing cell surface expression of SLC2A1			[3]
Role of PTM	Surface Expression Modulation
Related Enzyme	Serine/threonine-protein kinase SGK1 (SGK1)
Experimental Method	Co-Immunoprecipitation
Detailed Description	Deubiquitination at SLC2A1 have been reported to increase its cell surface expression.
N-glycosylation
Asparagine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC2A1			[4], [5]
Role of PTM	Potential impacts
Modified Residue	Asparagine	Modified Location	45
Experimental Method	Co-Immunoprecipitation
Detailed Description	N-linked Glycosylation at SLC2A1 Asparagine 45 has the potential to affect its expression or activity.
N-linked glycosylation
Unclear Residue	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	.			[6]
Role of PTM	Protein Stability
Experimental Material(s)	brain tissues
Experimental Method	Co-Immunoprecipitation
Detailed Description	N-linked glycosylation at SLC2A1 has been reported to increase its protein stability and expression levels in glioma.
O-glycosylation
Serine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC2A1			[7], [8]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	465
Experimental Method	Co-Immunoprecipitation
Detailed Description	O-linked Glycosylation at SLC2A1 Serine 465 has the potential to affect its expression or activity.
O-linked glycosylation
Unclear Residue	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	.			[9]
Role of PTM	Influencing the Disease Progression
Experimental Material(s)	BLCA tissue
Experimental Method	Co-Immunoprecipitation
Detailed Description	O-linked glycosylation at SLC2A1 has been reported to be a potential biomarker in bladder cancer due to its altered expression patterns in tumors.
Phosphorylation
Serine	10 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Enhancing the activity of SLC2A1			[10]
Role of PTM	Protein Activity Modulation
Affected Drug/Substrate	Glucose	Results for Drug	Increasing uptake of glucose
Modified Residue	Serine	Modified Location	226
Related Enzyme	Protein kinase C alpha type (PRKCA)
Studied Phenotype	Cervical cancer [ICD11: 2C77]
Experimental Material(s)	Human papillomavirus-related endocervical adenocarcinoma (HeLa) cells
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Serine 226 have been reported to enhance its transport activity.
PTM Phenomenon2	Have the potential to influence SLC2A1			[11], [12]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	4
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Serine 4 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC2A1			[11]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	5
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Serine 5 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC2A1			[13]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	113
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Serine 113 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence SLC2A1			[14]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	118
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Serine 118 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence SLC2A1			[15], [16]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	248
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Serine 248 has the potential to affect its expression or activity.
PTM Phenomenon7	Have the potential to influence SLC2A1			[17], [18]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	465
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Serine 465 has the potential to affect its expression or activity.
PTM Phenomenon8	Have the potential to influence SLC2A1			[18], [19]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	473
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Serine 473 has the potential to affect its expression or activity.
PTM Phenomenon9	Have the potential to influence SLC2A1			[18], [19]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	475
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Serine 475 has the potential to affect its expression or activity.
PTM Phenomenon10	Have the potential to influence SLC2A1			[17], [18]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	490
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Serine 490 has the potential to affect its expression or activity.
Threonine	5 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC2A1			[20], [21]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	234
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Threonine 234 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC2A1			[20], [21]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	238
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Threonine 238 has the potential to affect its expression or activity.
PTM Phenomenon3	.			[22]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	258
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Threonine 258 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC2A1			[21], [23]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	459
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Threonine 459 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence SLC2A1			[17], [24]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	478
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 Threonine 478 has the potential to affect its expression or activity.
Unclear Residue	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Increasing cell surface expression of SLC2A1			[3]
Role of PTM	Surface Expression Modulation
Related Enzyme	Serine/threonine-protein kinase SGK1 (SGK1)
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC2A1 have been reported to increase its cell surface expression.
S-palmitoylation
Cysteine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	.			[25]
Role of PTM	Trafficking to Plasma Membrane
Modified Residue	Cysteine	Modified Location	207
Experimental Material(s)	tumor tissue
Experimental Method	Co-Immunoprecipitation
Detailed Description	S-palmitoylation at SLC2A1 Cysteine 207 have been reported to increase its localization at the plasma membrane.
Sulfoxidation
Methionine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC2A1			[26]
Role of PTM	Potential impacts
Modified Residue	Methionine	Modified Location	244
Experimental Method	Co-Immunoprecipitation
Detailed Description	Sulfoxidation at SLC2A1 Methionine 244 has the potential to affect its expression or activity.
SUMOylation
Unclear Residue	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC2A1			[27]
Role of PTM	Potential impacts
Experimental Method	Co-Immunoprecipitation
Detailed Description	SUMOylation at SLC2A1 has the potential to affect its expression or activity.
Ubiquitination
Lysine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Decreasing cell surface expression of SLC2A1			[28]
Role of PTM	Surface Expression Modulation
Modified Residue	Lysine	Modified Location	245
Studied Phenotype	Hepatoblastoma [ICD11: 2C12.01]
Experimental Material(s)	Human hepatoblastoma (HepG2.2.15) cells
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC2A1 Lysine 245 have been reported to decrease its cell surface expression.
PTM Phenomenon2	Decreasing cell surface expression of SLC2A1			[28]
Role of PTM	Surface Expression Modulation
Modified Residue	Lysine	Modified Location	477
Studied Phenotype	Hepatoblastoma [ICD11: 2C12.01]
Experimental Material(s)	Human hepatoblastoma (HepG2.2.15) cells
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC2A1 Lysine 477 have been reported to decrease its cell surface expression.
Unclear Residue	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	.			[29]
Role of PTM	Degradation via Proteosome
Experimental Material(s)	tumor tissue
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC2A1 has been reported to promote its degradation.

References
1	Quantitative Proteomic Atlas of Ubiquitination and Acetylation in the DNA Damage Response. Mol Cell. 2015 Sep 3;59(5):867-81.
2	N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12449-54.
3	Proline Absorption and SGK1 Expression are Inhibited in Intestinal Tis7 Transgenic Mice. Cell Physiol Biochem. 2016;38(4):1532-43.
4	Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins. Nat Biotechnol. 2009 Apr;27(4):378-86.
5	The glut 1 glucose transporter interacts with calnexin and calreticulin. J Biol Chem. 1996 Jun 7;271(23):13691-6.
6	N-acetylglucosaminyltransferase I promotes glioma cell proliferation and migration through increasing the stability of the glucose transporter GLUT1. FEBS Lett. 2020;594(2):358-366.
7	Phosphoproteomic analysis of human embryonic stem cells. Cell Stem Cell. 2009 Aug 7;5(2):204-13.
8	Site-specific GlcNAcylation of human erythrocyte proteins: potential biomarker(s) for diabetes. Diabetes. 2009 Feb;58(2):309-17.
9	Aberrantly Glycosylated GLUT1 as a Poor Prognosis Marker in Aggressive Bladder Cancer. Int J Mol Sci. 2024;25(6):3462. Published 2024 Mar 19.
10	A Protein Kinase C Phosphorylation Motif in GLUT1 Affects Glucose Transport and is Mutated in GLUT1 Deficiency Syndrome. Mol Cell. 2015 Jun 4;58(5):845-53.
11	Tip-Based Fractionation of Batch-Enriched Phosphopeptides Facilitates Easy and Robust Phosphoproteome Analysis. J Proteome Res. 2018 Jan 5;17(1):46-54.
12	Insights into chemoselectivity principles in metal oxide affinity chromatography using tailored nanocast metal oxide microspheres and mass spectrometry-based phosphoproteomics. Analyst. 2017 May 30;142(11):1993-2003.
13	The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics. 2011 Jan;10(1):M110.004457.
14	Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3.
15	Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res. 2013 Jun 7;12(6):2414-21.
16	The Plasmodium falciparum schizont phosphoproteome reveals extensive phosphatidylinositol and cAMP-protein kinase A signaling. J Proteome Res. 2012 Nov 2;11(11):5323-37.
17	UniProt: a worldwide hub of protein knowledge. Nucleic Acids Res. 2019 Jan 8;47(D1):D506-D515.
18	Phosphoproteomic screening identifies physiological substrates of the CDKL5 kinase. EMBO J. 2018 Dec 14;37(24):e99559.
19	Integrated Proteomics Reveals Apoptosis-related Mechanisms Associated with Placental Malaria. Mol Cell Proteomics. 2019 Feb;18(2):182-199.
20	Defeating Major Contaminants in Fe3+- Immobilized Metal Ion Affinity Chromatography (IMAC) Phosphopeptide Enrichment. Mol Cell Proteomics. 2018 May;17(5):1028-1034.
21	CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-beta/BMP Signaling at the Primary Cilium. Cell Rep. 2018 Mar 6;22(10):2584-2592.
22	15 years of PhosphoSitePlus?: integrating post-translationally modified sites, disease variants and isoforms. Nucleic Acids Res. 2019;47(D1):D433-D441.
23	Proteogenomics connects somatic mutations to signalling in breast cancer. Nature. 2016 Jun 2;534(7605):55-62.
24	Detection of malignancy-associated phosphoproteome changes in human colorectal cancer induced by cell surface binding of growth-inhibitory galectin-4. IUBMB Life. 2019 Mar;71(3):364-375.
25	DHHC9-mediated GLUT1 S-palmitoylation promotes glioblastoma glycolysis and tumorigenesis. Nat Commun. 2021;12(1):5872. Published 2021 Oct 7.
26	Global analysis of methionine oxidation provides a census of folding stabilities for the human proteome. Proc Natl Acad Sci U S A. 2019 Mar 26;116(13):6081-6090.
27	Post-translational modifications of transporters. Pharmacol Ther. 2018 Dec;192:88-99.
28	Global analysis of HBV-mediated host proteome and ubiquitylome change in HepG2.2.15 human hepatoblastoma cell line. Cell Biosci. 2021 Apr 17;11(1):75.
29	TRIM38 triggers the uniquitination and degradation of glucose transporter type 1 (GLUT1) to restrict tumor progression in bladder cancer. J Transl Med. 2021;19(1):508. Published 2021 Dec 14.

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Detail Information of Post-Translational Modifications

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