Detail Information of Post-Translational Modifications

General Information of Drug Transporter (DT)
DT ID	DTD0511 Transporter Info
Gene Name	KCNJ11
Transporter Name	ATP-sensitive inward rectifier potassium channel 11
Gene ID	3767
UniProt ID	Q14654

Post-Translational Modification of This DT
Overview ofKCNJ11 Modification Sites with Functional and Structural Information
Sequence	M L S R K G I I P E E Y V L T R L A E D P A E P R Y R A R Q R R A R F V S K K G N C N V A H K N I R E Q G R F L Q D V F T T L V D L K W P H T L L I F T M S F L C S W L L F A M A W W L I A F A H G D L A P S E G T A E P C V T S I H S F S S A F L F S I E V Q V T I G F G G R M V T E E C P L A I L I L I V Q N I V G L M I N A I M L G C I F M K T A Q A H R R A E T L I F S K H A V I A L R H G R L C F M L R V G D L R K S M I I S A T I H M Q V V R K T T S P E G E V V P L H Q V D I P M E N G V G G N S I F L V A P L I I Y H V I D A N S P L Y D L A P S D L H H H Q D L E I I V I L E G V V E T T G I T T Q A R T S Y L A D E I L W G Q R F V P I V A E E D G R Y S V D Y S K F G N T I K V P T P L C T A R Q L D E D H S L L E A L T L A S A R G P L R K R S V P M A K A K P K F S I S P D S L S
PTM type	X-Oxidation X-Phosphorylation X-Phosphorylation X-Ubiquitination X: Amino Acid

Oxidation
Cystine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence KCNJ11			[1]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	42
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at KCNJ11 Cystine 42 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence KCNJ11			[1]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	344
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at KCNJ11 Cystine 344 has the potential to affect its expression or activity.
Phosphorylation
Serine	6 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence KCNJ11			[2]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	184
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Serine 184 has the potential to affect its expression or activity.
PTM Phenomenon2	.			[3]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	208
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Serine 208 has the potential to affect its expression or activity.
PTM Phenomenon3	.			[4]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	285
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Serine 285 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence KCNJ11			[2], [5]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	372
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Serine 372 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence KCNJ11			[6], [7]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	385
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Serine 385 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence KCNJ11			[8]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	388
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Serine 388 has the potential to affect its expression or activity.
Threonine	8 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	.			[4]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	93
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Threonine 93 has the potential to affect its expression or activity.
PTM Phenomenon2	.			[4]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	137
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Threonine 137 has the potential to affect its expression or activity.
PTM Phenomenon3	.			[4]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	137
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Threonine 137 has the potential to affect its expression or activity.
PTM Phenomenon4	.			[4]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	137
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Threonine 137 has the potential to affect its expression or activity.
PTM Phenomenon5	.			[3]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	180
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Threonine 180 has the potential to affect its expression or activity.
PTM Phenomenon6	.			[3]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	224
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Threonine 224 has the potential to affect its expression or activity.
PTM Phenomenon7	Have the potential to influence KCNJ11			[9]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	298
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Threonine 298 has the potential to affect its expression or activity.
PTM Phenomenon8	Have the potential to influence KCNJ11			[6], [9]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	341
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Threonine 341 has the potential to affect its expression or activity.
Tyrosine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence KCNJ11			[10]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	26
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 Tyrosine 26 has the potential to affect its expression or activity.
Unclear Residue	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Affecting the stability and reducing the activity of KCNJ11			[9]
Role of PTM	Protein Stability
Related Enzyme	Mitogen-activated protein kinase 1 (MAPK1)
Experimental Material(s)	Human embryonic kidney 293 (HEK293) cells
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at KCNJ11 have been reported to affect its stability and reduce its transport activity.
Ubiquitination
Lysine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence KCNJ11			[11]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	332
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at KCNJ11 Lysine 332 has the potential to affect its expression or activity.

References
1	A Quantitative Tissue-Specific Landscape of Protein Redox Regulation during Aging. Cell. 2020 Mar 5;180(5):968-983.e24.
2	Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics. 2014 Jul;13(7):1690-704.
3	15 years of PhosphoSitePlus?: integrating post-translationally modified sites, disease variants and isoforms. Nucleic Acids Res. 2019;47(D1):D433-D441.
4	ActiveDriverDB: human disease mutations and genome variation in post-translational modification sites of proteins. Nucleic Acids Res. 2018;46(D1):D901-D910.
5	Global Phosphoproteomic Analysis of Human Skeletal Muscle Reveals a Network of Exercise-Regulated Kinases and AMPK Substrates. Cell Metab. 2015 Nov 3;22(5):922-35.
6	UniProt: a worldwide hub of protein knowledge. Nucleic Acids Res. 2019 Jan 8;47(D1):D506-D515.
7	Conserved functional consequences of disease-associated mutations in the slide helix of Kir6.1 and Kir6.2 subunits of the ATP-sensitive potassium channel. J Biol Chem. 2017 Oct 20;292(42):17387-17398.
8	Proteogenomic integration reveals therapeutic targets in breast cancer xenografts. Nat Commun. 2017 Mar 28;8:14864.
9	Functional modulation of the ATP-sensitive potassium channel by extracellular signal-regulated kinase-mediated phosphorylation. Neuroscience. 2008 Mar 18;152(2):371-80.
10	Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. Cell. 2007 Dec 14;131(6):1190-203.
11	A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. Mol Cell Proteomics. 2011 Oct;10(10):M111.013284.

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Detail Information of Post-Translational Modifications

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