Detail Information of Post-Translational Modifications

General Information of Drug Transporter (DT)
DT ID	DTD0403 Transporter Info
Gene Name	SLC56A1
Transporter Name	Sideroflexin-1
Gene ID	94081
UniProt ID	Q9H9B4

Post-Translational Modification of This DT
Overview ofSLC56A1 Modification Sites with Functional and Structural Information
Sequence	M S G E L P P N I N I K E P R W D Q S T F I G R A N H F F T V T D P R N I L L T N E Q L E S A R K I V H D Y R Q G I V P P G L T E N E L W R A K Y I Y D S A F H P D T G E K M I L I G R M S A Q V P M N M T I T G C M M T F Y R T T P A V L F W Q W I N Q S F N A V V N Y T N R S G D A P L T V N E L G T A Y V S A T T G A V A T A L G L N A L T K H V S P L I G R F V P F A A V A A A N C I N I P L M R Q R E L K V G I P V T D E N G N R L G E S A N A A K Q A I T Q V V V S R I L M A A P G M A I P P F I M N T L E K K A F L K R F P W M S A P I Q V G L V G F C L V F A T P L C C A L F P Q K S S M S V T S L E A E L Q A K I Q E S H P E L R R V Y F N K G L
PTM type	X-Acetylation X-Methylation X-Oxidation X-Phosphorylation X-S-nitrosylation X-Sulfoxidation X-Ubiquitination X-Ubiquitination X: Amino Acid

Acetylation
Lysine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[1], [2]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	72
Experimental Method	Co-Immunoprecipitation
Detailed Description	Acetylation at SLC56A1 Lysine 72 has the potential to affect its expression or activity.
Serine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[3], [4]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	2
Experimental Method	Co-Immunoprecipitation
Detailed Description	Acetylation at SLC56A1 Serine 2 has the potential to affect its expression or activity.
Methylation
Arginine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[5]
Role of PTM	Potential impacts
Modified Residue	Arginine	Modified Location	314
Experimental Method	Co-Immunoprecipitation
Detailed Description	Methylation at SLC56A1 Arginine 314 has the potential to affect its expression or activity.
Oxidation
Cystine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SFXN1			[6]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	106
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at SFXN1 Cystine 106 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SFXN1			[6]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	190
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at SFXN1 Cystine 190 has the potential to affect its expression or activity.
Phosphorylation
Serine	11 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[7]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	2
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 2 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC56A1			[8], [9]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	19
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 19 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC56A1			[10]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	46
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 46 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC56A1			[11]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	77
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 77 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence SLC56A1			[12], [13]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	173
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 173 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence SLC56A1			[14]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	218
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 218 has the potential to affect its expression or activity.
PTM Phenomenon7	Have the potential to influence SLC56A1			[8], [15]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	232
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 232 has the potential to affect its expression or activity.
PTM Phenomenon8	Have the potential to influence SLC56A1			[16], [17]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	291
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 291 has the potential to affect its expression or activity.
PTM Phenomenon9	Have the potential to influence SLC56A1			[16], [17]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	292
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 292 has the potential to affect its expression or activity.
PTM Phenomenon10	Have the potential to influence SLC56A1			[16], [18]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	294
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 294 has the potential to affect its expression or activity.
PTM Phenomenon11	Have the potential to influence SLC56A1			[17], [18]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	297
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Serine 297 has the potential to affect its expression or activity.
Threonine	3 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[8]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	20
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Threonine 20 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC56A1			[8]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	227
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Threonine 227 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC56A1			[17], [18]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	296
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Threonine 296 has the potential to affect its expression or activity.
Tyrosine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[11]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	73
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Tyrosine 73 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC56A1			[11], [19]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	75
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC56A1 Tyrosine 75 has the potential to affect its expression or activity.
S-nitrosylation
Cystine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SFXN1			[20]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	190
Experimental Method	Co-Immunoprecipitation
Detailed Description	S-nitrosylation (-SNO) at SFXN1 Cystine 190 has the potential to affect its expression or activity.
Sulfoxidation
Methionine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[21]
Role of PTM	Potential impacts
Modified Residue	Methionine	Modified Location	293
Experimental Method	Co-Immunoprecipitation
Detailed Description	Sulfoxidation at SLC56A1 Methionine 293 has the potential to affect its expression or activity.
Ubiquitination
Alanine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[12], [22]
Role of PTM	Potential impacts
Modified Residue	Alanine	Modified Location	25
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Alanine 25 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC56A1			[23], [24]
Role of PTM	Potential impacts
Modified Residue	Alanine	Modified Location	162
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Alanine 162 has the potential to affect its expression or activity.
Isoleucine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[25], [26]
Role of PTM	Potential impacts
Modified Residue	Isoleucine	Modified Location	11
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Isoleucine 11 has the potential to affect its expression or activity.
Lysine	8 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[27]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	12
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Lysine 12 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC56A1			[23], [24]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	49
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Lysine 49 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC56A1			[24], [25]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	72
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Lysine 72 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC56A1			[12], [22]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	86
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Lysine 86 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence SLC56A1			[24], [25]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	202
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Lysine 202 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence SLC56A1			[23], [24]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	223
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Lysine 223 has the potential to affect its expression or activity.
PTM Phenomenon7	Have the potential to influence SLC56A1			[24], [28]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	305
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Lysine 305 has the potential to affect its expression or activity.
PTM Phenomenon8	.			[29]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	320
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SFXN1 Lysine 320 has the potential to affect its expression or activity.
Proline	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC56A1			[24], [25]
Role of PTM	Potential impacts
Modified Residue	Proline	Modified Location	141
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Proline 141 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC56A1			[24], [28]
Role of PTM	Potential impacts
Modified Residue	Proline	Modified Location	244
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC56A1 Proline 244 has the potential to affect its expression or activity.

References
1	Lysine Acetylation and Succinylation in HeLa Cells and their Essential Roles in Response to UV-induced Stress. Sci Rep. 2016 Jul 25;6:30212.
2	Suberoylanilide hydroxamic acid treatment reveals crosstalks among proteome, ubiquitylome and acetylome in non-small cell lung cancer A549 cell line. Sci Rep. 2015 Mar 31;5:9520.
3	Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501.
4	N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12449-54.
5	dbPTM in 2022: an updated database for exploring regulatory networks and functional associations of protein post-translational modifications. Nucleic Acids Res. 2022 Jan 7;50(D1):D471-D479. (ID: SFXN1_HUMAN)
6	A Quantitative Tissue-Specific Landscape of Protein Redox Regulation during Aging. Cell. 2020 Mar 5;180(5):968-983.e24.
7	Phosphoproteomics to Characterize Host Response During Influenza A Virus Infection of Human Macrophages. Mol Cell Proteomics. 2016 Oct;15(10):3203-3219.
8	Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics. 2014 Jul;13(7):1690-704.
9	An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62.
10	Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3.
11	Deep Phosphotyrosine Proteomics by Optimization of Phosphotyrosine Enrichment and MS/MS Parameters. J Proteome Res. 2017 Feb 3;16(2):1077-1086.
12	Systematic functional prioritization of protein posttranslational modifications. Cell. 2012 Jul 20;150(2):413-25.
13	System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3.
14	Deep Coverage of Global Protein Expression and Phosphorylation in Breast Tumor Cell Lines Using TMT 10-plex Isobaric Labeling. J Proteome Res. 2017 Mar 3;16(3):1121-1132.
15	Quantitative phosphoproteomics identifies substrates and functional modules of Aurora and Polo-like kinase activities in mitotic cells. Sci Signal. 2011 Jun 28;4(179):rs5.
16	Identification of Missing Proteins in the Phosphoproteome of Kidney Cancer. J Proteome Res. 2017 Dec 1;16(12):4364-4373.
17	Proteogenomics connects somatic mutations to signalling in breast cancer. Nature. 2016 Jun 2;534(7605):55-62.
18	Proteogenomic integration reveals therapeutic targets in breast cancer xenografts. Nat Commun. 2017 Mar 28;8:14864.
19	Phosphoproteomics Analysis Identifies Novel Candidate Substrates of the Nonreceptor Tyrosine Kinase, S rc- r elated Kinase Lacking C-terminal Regulatory Tyrosine and N-terminal M yristoylation S ites (SRMS). Mol Cell Proteomics. 2018 May;17(5):925-947.
20	Dual Labeling Biotin Switch Assay to Reduce Bias Derived From Different Cysteine Subpopulations: A Method to Maximize S-Nitrosylation Detection. Circ Res. 2015 Oct 23;117(10):846-57.
21	Redox-based reagents for chemoselective methionine bioconjugation. Science. 2017 Feb 10;355(6325):597-602.
22	Global identification of modular cullin-RING ligase substrates. Cell. 2011 Oct 14;147(2):459-74.
23	Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature. 2021 Jun;594(7862):246-252.
24	UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol. 2018 Jul;25(7):631-640.
25	Highly Multiplexed Quantitative Mass Spectrometry Analysis of Ubiquitylomes. Cell Syst. 2016 Oct 26;3(4):395-403.e4.
26	Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization. Nature. 2013 Apr 18;496(7445):372-6.
27	A COFRADIC protocol to study protein ubiquitination. J Proteome Res. 2014 Jun 6;13(6):3107-13.
28	Global site-specific neddylation profiling reveals that NEDDylated cofilin regulates actin dynamics. Nat Struct Mol Biol. 2020 Feb;27(2):210-220.
29	ActiveDriverDB: human disease mutations and genome variation in post-translational modification sites of proteins. Nucleic Acids Res. 2018;46(D1):D901-D910.

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Detail Information of Post-Translational Modifications

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