Detail Information of Post-Translational Modifications

General Information of Drug Transporter (DT)
DT ID	DTD0477 Transporter Info
Gene Name	SLC8A1
Transporter Name	Sodium/calcium exchanger 1
Gene ID	6546
UniProt ID	P32418

Post-Translational Modification of This DT
Overview ofSLC8A1 Modification Sites with Functional and Structural Information
Sequence	M Y N M R R L S L S P T F S M G F H L L V T V S L L F S H V D H V I A E T E M E G E G N E T G E C T G S Y Y C K K G V I L P I W E P Q D P S F G D K I A R A T V Y F V A M V Y M F L G V S I I A D R F M S S I E V I T S Q E K E I T I K K P N G E T T K T T V R I W N E T V S N L T L M A L G S S A P E I L L S V I E V C G H N F T A G D L G P S T I V G S A A F N M F I I I A L C V Y V V P D G E T R K I K H L R V F F V T A A W S I F A Y T W L Y I I L S V I S P G V V E V W E G L L T F F F F P I C V V F A W V A D R R L L F Y K Y V Y K R Y R A G K Q R G M I I E H E G D R P S S K T E I E M D G K V V N S H V E N F L D G A L V L E V D E R D Q D D E E A R R E M A R I L K E L K Q K H P D K E I E Q L I E L A N Y Q V L S Q Q Q K S R A F Y R I Q A T R L M T G A G N I L K R H A A D Q A R K A V S M H E V N T E V T E N D P V S K I F F E Q G T Y Q C L E N C G T V A L T I I R R G G D L T N T V F V D F R T E D G T A N A G S D Y E F T E G T V V F K P G D T Q K E I R V G I I D D D I F E E D E N F L V H L S N V K V S S E A S E D G I L E A N H V S T L A C L G S P S T A T V T I F D D D H A G I F T F E E P V T H V S E S I G I M E V K V L R T S G A R G N V I V P Y K T I E G T A R G G G E D F E D T C G E L E F Q N D E I V K T I S V K V I D D E E Y E K N K T F F L E I G E P R L V E M S E K K A L L L N E L G G F T I T G K Y L F G Q P V F R K V H A R E H P I L S T V I T I A D E Y D D K Q P L T S K E E E E R R I A E M G R P I L G E H T K L E V I I E E S Y E F K S T V D K L I K K T N L A L V V G T N S W R E Q F I E A I T V S A G E D D D D D E C G E E K L P S C F D Y V M H F L T V F W K V L F A F V P P T E Y W N G W A C F I V S I L M I G L L T A F I G D L A S H F G C T I G L K D S V T A V V F V A L G T S V P D T F A S K V A A T Q D Q Y A D A S I G N V T G S N A V N V F L G I G V A W S I A A I Y H A A N G E Q F K V S P G T L A F S V T L F T I F A F I N V G V L L Y R R R P E I G G E L G G P R T A K L L T S C L F V L L W L L Y I F F S S L E A Y C H I K G F
PTM type	X-N-glycosylation X-Oxidation X-Palmitoylation X-Phosphorylation X-Phosphorylation X: Amino Acid

N-glycosylation
Asparagine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC8A1			[1]
Role of PTM	Potential impacts
Modified Residue	Asparagine	Modified Location	44
Experimental Method	Co-Immunoprecipitation
Detailed Description	N-linked Glycosylation at SLC8A1 Asparagine 44 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC8A1			[1]
Role of PTM	Potential impacts
Modified Residue	Asparagine	Modified Location	160
Experimental Method	Co-Immunoprecipitation
Detailed Description	N-linked Glycosylation at SLC8A1 Asparagine 160 has the potential to affect its expression or activity.
Oxidation
Cystine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC8A1			[2]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	592
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at SLC8A1 Cystine 592 has the potential to affect its expression or activity.
Palmitoylation
Cysteine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Increasing the participation of SLC8A1 in membrane domain-dependent endocytosis			[3]
Role of PTM	Surface Expression Modulation
Modified Residue	Cysteine	Modified Location	739
Experimental Method	Co-Immunoprecipitation
Detailed Description	Palmitoylation at SLC8A1 Cysteine 739 have been reported to increase its participation in membrane domain-dependent endocytosis.
Phosphorylation
Serine	10 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC8A1			[4]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	93
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Serine 93 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC8A1			[5]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	101
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Serine 101 has the potential to affect its expression or activity.
PTM Phenomenon3	.			[6]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	145
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Serine 145 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC8A1			[7], [8]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	284
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Serine 284 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence SLC8A1			[7], [8]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	285
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Serine 285 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence SLC8A1			[8], [9]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	392
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Serine 392 has the potential to affect its expression or activity.
PTM Phenomenon7	Have the potential to influence SLC8A1			[5], [10]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	407
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Serine 407 has the potential to affect its expression or activity.
PTM Phenomenon8	Have the potential to influence SLC8A1			[11]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	455
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Serine 455 has the potential to affect its expression or activity.
PTM Phenomenon9	Have the potential to influence SLC8A1			[12], [13]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	690
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Serine 690 has the potential to affect its expression or activity.
PTM Phenomenon10	.			[14]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	724
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Serine 724 has the potential to affect its expression or activity.
Threonine	8 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC8A1			[5]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	107
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Threonine 107 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC8A1			[9]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	122
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Threonine 122 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC8A1			[9]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	123
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Threonine 123 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC8A1			[7], [11]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	287
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Threonine 287 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence SLC8A1			[11], [15]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	398
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Threonine 398 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence SLC8A1			[10], [16]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	401
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Threonine 401 has the potential to affect its expression or activity.
PTM Phenomenon7	Have the potential to influence SLC8A1			[12], [13]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	689
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Threonine 689 has the potential to affect its expression or activity.
PTM Phenomenon8	.			[14]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	725
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Threonine 725 has the potential to affect its expression or activity.
Tyrosine	4 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC8A1			[17]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	259
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Tyrosine 259 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC8A1			[17], [18]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	261
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Tyrosine 261 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC8A1			[19]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	351
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Tyrosine 351 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC8A1			[19]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	574
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC8A1 Tyrosine 574 has the potential to affect its expression or activity.

References
1	dbPTM in 2022: an updated database for exploring regulatory networks and functional associations of protein post-translational modifications. Nucleic Acids Res. 2022 Jan 7;50(D1):D471-D479. (ID: NAC1_HUMAN)
2	A Quantitative Tissue-Specific Landscape of Protein Redox Regulation during Aging. Cell. 2020 Mar 5;180(5):968-983.e24.
3	Palmitoylation of the Na/Ca exchanger cytoplasmic loop controls its inactivation and internalization during stress signaling. FASEB J. 2015 Nov;29(11):4532-43.
4	Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics. 2014 Jul;13(7):1690-704.
5	A fast sample processing strategy for large-scale profiling of human urine phosphoproteome by mass spectrometry. Talanta. 2018 Aug 1;185:166-173.
6	15 years of PhosphoSitePlus?: integrating post-translationally modified sites, disease variants and isoforms. Nucleic Acids Res. 2019;47(D1):D433-D441.
7	Defeating Major Contaminants in Fe3+- Immobilized Metal Ion Affinity Chromatography (IMAC) Phosphopeptide Enrichment. Mol Cell Proteomics. 2018 May;17(5):1028-1034.
8	CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-beta/BMP Signaling at the Primary Cilium. Cell Rep. 2018 Mar 6;22(10):2584-2592.
9	An integrated strategy for highly sensitive phosphoproteome analysis from low micrograms of protein samples. Analyst. 2018 Jul 23;143(15):3693-3701.
10	Phosphoproteomics to Characterize Host Response During Influenza A Virus Infection of Human Macrophages. Mol Cell Proteomics. 2016 Oct;15(10):3203-3219.
11	Identification of Missing Proteins in the Phosphoproteome of Kidney Cancer. J Proteome Res. 2017 Dec 1;16(12):4364-4373.
12	Integrative network analysis of signaling in human CD34(+) hematopoietic progenitor cells by global phosphoproteomic profiling using TiO2 enrichment combined with 2D LC-MS/MS and pathway mapping. Proteomics. 2013 Apr;13(8):1325-33.
13	Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep. 2017 Mar 28;18(13):3242-3256.
14	ActiveDriverDB: human disease mutations and genome variation in post-translational modification sites of proteins. Nucleic Acids Res. 2018;46(D1):D901-D910.
15	Phosphoproteome Analysis Reveals Differential Mode of Action of Sorafenib in Wildtype and Mutated FLT3 Acute Myeloid Leukemia (AML) Cells. Mol Cell Proteomics. 2017 Jul;16(7):1365-1376.
16	Phosphoproteomics reveals ALK promote cell progress via RAS/ JNK pathway in neuroblastoma. Oncotarget. 2016 Nov 15;7(46):75968-75980.
17	Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. Cell. 2007 Dec 14;131(6):1190-203.
18	Systematic functional prioritization of protein posttranslational modifications. Cell. 2012 Jul 20;150(2):413-25.
19	Neuroblastoma tyrosine kinase signaling networks involve FYN and LYN in endosomes and lipid rafts. PLoS Comput Biol. 2015 Apr 17;11(4):e1004130.

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Detail Information of Post-Translational Modifications

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