Detail Information of Post-Translational Modifications

General Information of Drug Transporter (DT)
DT ID	DTD0170 Transporter Info
Gene Name	SLC25A12
Transporter Name	Calcium-binding mitochondrial carrier protein Aralar1
Gene ID	8604
UniProt ID	O75746

Post-Translational Modification of This DT
Overview ofSLC25A12 Modification Sites with Functional and Structural Information
Sequence	M A V K V Q T T K R G D P H E L R N I F L Q Y A S T E V D G E R Y M T P E D F V Q R Y L G L Y N D P N S N P K I V Q L L A G V A D Q T K D G L I S Y Q E F L A F E S V L C A P D S M F I V A F Q L F D K S G N G E V T F E N V K E I F G Q T I I H H H I P F N W D C E F I R L H F G H N R K K H L N Y T E F T Q F L Q E L Q L E H A R Q A F A L K D K S K S G M I S G L D F S D I M V T I R S H M L T P F V E E N L V S A A G G S I S H Q V S F S Y F N A F N S L L N N M E L V R K I Y S T L A G T R K D V E V T K E E F A Q S A I R Y G Q V T P L E I D I L Y Q L A D L Y N A S G R L T L A D I E R I A P L A E G A L P Y N L A E L Q R Q Q S P G L G R P I W L Q I A E S A Y R F T L G S V A G A V G A T A V Y P I D L V K T R M Q N Q R G S G S V V G E L M Y K N S F D C F K K V L R Y E G F F G L Y R G L I P Q L I G V A P E K A I K L T V N D F V R D K F T R R D G S V P L P A E V L A G G C A G G S Q V I F T N P L E I V K I R L Q V A G E I T T G P R V S A L N V L R D L G I F G L Y K G A K A C F L R D I P F S A I Y F P V Y A H C K L L L A D E N G H V G G L N L L A A G A M A G V P A A S L V T P A D V I K T R L Q V A A R A G Q T T Y S G V I D C F R K I L R E E G P S A F W K G T A A R V F R S S P Q F G V T L V T Y E L L Q R W F Y I D F G G L K P A G S E P T P K S R I A D L P P A N P D H I G G Y R L A T A T F A G I E N K F G L Y L P K F K S P S V A V V Q P K A A V A A T Q
PTM type	X-Acetylation X-Methylation X-O-glycosylation X-Oxidation X-Phosphorylation X-Phosphorylation X-S-nitrosylation X-Ubiquitination X: Amino Acid

Acetylation
Alanine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[1], [2]
Role of PTM	Potential impacts
Modified Residue	Alanine	Modified Location	2
Experimental Method	Co-Immunoprecipitation
Detailed Description	Acetylation at SLC25A12 Alanine 2 has the potential to affect its expression or activity.
Lysine	3 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[3]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	112
Experimental Method	Co-Immunoprecipitation
Detailed Description	Acetylation at SLC25A12 Lysine 112 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC25A12			[4], [5]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	377
Experimental Method	Co-Immunoprecipitation
Detailed Description	Acetylation at SLC25A12 Lysine 377 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC25A12			[6]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	578
Experimental Method	Co-Immunoprecipitation
Detailed Description	Acetylation at SLC25A12 Lysine 578 has the potential to affect its expression or activity.
Methylation
Lysine	3 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[7]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	377
Experimental Method	Co-Immunoprecipitation
Detailed Description	Methylation at SLC25A12 Lysine 377 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC25A12			[8]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	451
Experimental Method	Co-Immunoprecipitation
Detailed Description	Methylation at SLC25A12 Lysine 451 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC25A12			[8]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	661
Experimental Method	Co-Immunoprecipitation
Detailed Description	Methylation at SLC25A12 Lysine 661 has the potential to affect its expression or activity.
O-glycosylation
Serine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[9], [10]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	256
Experimental Method	Co-Immunoprecipitation
Detailed Description	O-linked Glycosylation at SLC25A12 Serine 256 has the potential to affect its expression or activity.
Oxidation
Cystine	4 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[11]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	130
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at SLC25A12 Cystine 130 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC25A12			[11]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	375
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at SLC25A12 Cystine 375 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC25A12			[11]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	505
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at SLC25A12 Cystine 505 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC25A12			[12]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	563
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at SLC25A12 Cystine 563 has the potential to affect its expression or activity.
Phosphorylation
Serine	6 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[13], [14]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	101
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Serine 101 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC25A12			[13], [15]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	237
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Serine 237 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC25A12			[16]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	281
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Serine 281 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC25A12			[17]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	360
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Serine 360 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence SLC25A12			[13], [14]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	662
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Serine 662 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence SLC25A12			[13], [14]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	664
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Serine 664 has the potential to affect its expression or activity.
Threonine	9 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	.			[18]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	188
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Threonine 188 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC25A12			[13], [15]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	238
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Threonine 238 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC25A12			[13], [19]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	242
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Threonine 242 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC25A12			[20]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	461
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Threonine 461 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence SLC25A12			[21]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	537
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Threonine 537 has the potential to affect its expression or activity.
PTM Phenomenon6	.			[18]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	643
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Threonine 643 has the potential to affect its expression or activity.
PTM Phenomenon7	.			[18]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	643
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Threonine 643 has the potential to affect its expression or activity.
PTM Phenomenon8	.			[18]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	643
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Threonine 643 has the potential to affect its expression or activity.
PTM Phenomenon9	.			[18]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	643
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Threonine 643 has the potential to affect its expression or activity.
Tyrosine	3 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[15]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	236
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Tyrosine 236 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC25A12			[16]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	278
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Tyrosine 278 has the potential to affect its expression or activity.
PTM Phenomenon3	.			[18]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	656
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC25A12 Tyrosine 656 has the potential to affect its expression or activity.
S-nitrosylation
Cystine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[22]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	375
Experimental Method	Co-Immunoprecipitation
Detailed Description	S-nitrosylation (-SNO) at SLC25A12 Cystine 375 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC25A12			[22]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	563
Experimental Method	Co-Immunoprecipitation
Detailed Description	S-nitrosylation (-SNO) at SLC25A12 Cystine 563 has the potential to affect its expression or activity.
Ubiquitination
Cystine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[23]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	375
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Cystine 375 has the potential to affect its expression or activity.
Glycine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[23]
Role of PTM	Potential impacts
Modified Residue	Glycine	Modified Location	391
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Glycine 391 has the potential to affect its expression or activity.
Leucine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[23]
Role of PTM	Potential impacts
Modified Residue	Leucine	Modified Location	315
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Leucine 315 has the potential to affect its expression or activity.
Lysine	7 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[24]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	4
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Lysine 4 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC25A12			[25]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	55
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Lysine 55 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC25A12			[26]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	250
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Lysine 250 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC25A12			[27]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	377
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Lysine 377 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence SLC25A12			[23]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	406
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Lysine 406 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence SLC25A12			[23], [25]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	482
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Lysine 482 has the potential to affect its expression or activity.
PTM Phenomenon7	Have the potential to influence SLC25A12			[23], [27]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	485
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Lysine 485 has the potential to affect its expression or activity.
Proline	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC25A12			[23]
Role of PTM	Potential impacts
Modified Residue	Proline	Modified Location	394
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at SLC25A12 Proline 394 has the potential to affect its expression or activity.

References
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2	Biochemical and cellular analysis of Ogden syndrome reveals downstream Nt-acetylation defects. Hum Mol Genet. 2015 Apr 1;24(7):1956-76.
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5	Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells. Sci Rep. 2016 Jan 29;6:19926.
6	Potent Antagonists of RORgamat, Cardenolides from Calotropis gigantea, Exhibit Discrepant Effects on the Differentiation of T Lymphocyte Subsets. Mol Pharm. 2019 Feb 4;16(2):798-807.
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9	A triarylphosphine-trimethylpiperidine reagent for the one-step derivatization and enrichment of protein post-translational modifications and identification by mass spectrometry. Chem Commun (Camb). 2018 Dec 6;54(98):13790-13793.
10	Synthesis of a Highly Azide-Reactive and Thermosensitive Biofunctional Reagent for Efficient Enrichment and Large-Scale Identification of O-GlcNAc Proteins by Mass Spectrometry. Anal Chem. 2017 Jun 6;89(11):5810-5817.
11	A Quantitative Tissue-Specific Landscape of Protein Redox Regulation during Aging. Cell. 2020 Mar 5;180(5):968-983.e24.
12	Identifying Functional Cysteine Residues in the Mitochondria. ACS Chem Biol. 2017 Apr 21;12(4):947-957.
13	Global Phosphoproteomic Analysis of Human Skeletal Muscle Reveals a Network of Exercise-Regulated Kinases and AMPK Substrates. Cell Metab. 2015 Nov 3;22(5):922-35.
14	Insulin increases phosphorylation of mitochondrial proteins in human skeletal muscle in vivo. J Proteome Res. 2014 May 2;13(5):2359-69.
15	System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3.
16	Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun. 2012 Jun 6;3:876.
17	Identification of Missing Proteins in the Phosphoproteome of Kidney Cancer. J Proteome Res. 2017 Dec 1;16(12):4364-4373.
18	ActiveDriverDB: human disease mutations and genome variation in post-translational modification sites of proteins. Nucleic Acids Res. 2018;46(D1):D901-D910.
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22	Dual Labeling Biotin Switch Assay to Reduce Bias Derived From Different Cysteine Subpopulations: A Method to Maximize S-Nitrosylation Detection. Circ Res. 2015 Oct 23;117(10):846-57.
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24	Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Mol Cell Proteomics. 2012 May;11(5):148-59.
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27	Global Landscape and Dynamics of Parkin and USP30-Dependent Ubiquitylomes in iNeurons during Mitophagic Signaling. Mol Cell. 2020 Mar 5;77(5):1124-1142.e10.

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Detail Information of Post-Translational Modifications

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