Detail Information of Post-Translational Modifications

General Information of Drug Transporter (DT)
DT ID	DTD0009 Transporter Info
Gene Name	SLC22A3
Transporter Name	Organic cation transporter 3
Gene ID	6581
UniProt ID	O75751

Post-Translational Modification of This DT
Overview ofSLC22A3 Modification Sites with Functional and Structural Information
Sequence	M P S F D E A L Q R V G E F G R F Q R R V F L L L C L T G V T F A F L F V G V V F L G T Q P D H Y W C R G P S A A A L A E R C G W S P E E E W N R T A P A S R G P E P P E R R G R C Q R Y L L E A A N D S A S A T S A L S C A D P L A A F P N R S A P L V P C R G G W R Y A Q A H S T I V S E F D L V C V N A W M L D L T Q A I L N L G F L T G A F T L G Y A A D R Y G R I V I Y L L S C L G V G V T G V V V A F A P N F P V F V I F R F L Q G V F G K G T W M T C Y V I V T E I V G S K Q R R I V G I V I Q M F F T L G I I I L P G I A Y F I P N W Q G I Q L A I T L P S F L F L L Y Y W V V P E S P R W L I T R K K G D K A L Q I L R R I A K C N G K Y L S S N Y S E I T V T D E E V S N P S F L D L V R T P Q M R K C T L I L M F A W F T S A V V Y Q G L V M R L G I I G G N L Y I D F F I S G V V E L P G A L L I L L T I E R L G R R L P F A A S N I V A G V A C L V T A F L P E G I A W L R T T V A T L G R L G I T M A F E I V Y L V N S E L Y P T T L R N F G V S L C S G L C D F G G I I A P F L L F R L A A V W L E L P L I I F G I L A S I C G G L V M L L P E T K G I A L P E T V D D V E K L G S P H S C K C G R N K K T P V S R S H L
PTM type	X-N-glycosylation X-Phosphorylation X: Amino Acid

N-glycosylation
Asparagine	4 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC22A3			[1]
Role of PTM	Potential impacts
Modified Residue	Asparagine	Modified Location	72
Experimental Method	Co-Immunoprecipitation
Detailed Description	N-linked Glycosylation at SLC22A3 Asparagine 72 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC22A3			[1]
Role of PTM	Potential impacts
Modified Residue	Asparagine	Modified Location	99
Experimental Method	Co-Immunoprecipitation
Detailed Description	N-linked Glycosylation at SLC22A3 Asparagine 99 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC22A3			[1]
Role of PTM	Potential impacts
Modified Residue	Asparagine	Modified Location	119
Experimental Method	Co-Immunoprecipitation
Detailed Description	N-linked Glycosylation at SLC22A3 Asparagine 119 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC22A3			[1]
Role of PTM	Potential impacts
Modified Residue	Asparagine	Modified Location	322
Experimental Method	Co-Immunoprecipitation
Detailed Description	N-linked Glycosylation at SLC22A3 Asparagine 322 has the potential to affect its expression or activity.
Phosphorylation
Serine	4 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC22A3			[2]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	78
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A3 Serine 78 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC22A3			[3]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	334
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A3 Serine 334 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC22A3			[3]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	337
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A3 Serine 337 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC22A3			[4]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	537
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A3 Serine 537 has the potential to affect its expression or activity.
Threonine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC22A3			[3]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	327
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A3 Threonine 327 has the potential to affect its expression or activity.
Tyrosine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Potentially affecting the transporter			[5], [6]
Role of PTM	On/Off Switch
Modified Residue	Tyrosine
Modified State	Tyrosine kinase inhibitors (Pazopanib)
Experimental Method	Co-Immunoprecipitation
Detailed Description	Removal of the Phosphorylation at SLC22A3 Tyrosine (i.e. Tyrosine kinase inhibitors (Pazopanib)) have been reported to have the potential to affect the transporter.
PTM Phenomenon2	Have the potential to influence SLC22A3			[3]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	318
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A3 Tyrosine 318 has the potential to affect its expression or activity.

References
1	dbPTM in 2022: an updated database for exploring regulatory networks and functional associations of protein post-translational modifications. Nucleic Acids Res. 2022 Jan 7;50(D1):D471-D479. (ID: S22A3_HUMAN)
2	Synthesizing Signaling Pathways from Temporal Phosphoproteomic Data. Cell Rep. 2018 Sep 25;24(13):3607-3618.
3	Mass spectrometric phosphoproteome analysis of small-sized samples of human neutrophils. Clin Chim Acta. 2015 Dec 7;451(Pt B):199-207.
4	Phosphoproteomics Reveals MAPK Inhibitors Enhance MET- and EGFR-Driven AKT Signaling in KRAS-Mutant Lung Cancer. Mol Cancer Res. 2016 Oct;14(10):1019-1029.
5	Inhibition of OCT2, MATE1 and MATE2-K as a possible mechanism of drug interaction between pazopanib and cisplatin. Pharmacol Res. 2016 Aug;110:89-95.
6	A phosphotyrosine switch regulates organic cation transporters. Nat Commun. 2016 Mar 16;7:10880.

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Detail Information of Post-Translational Modifications

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