Detail Information of Post-Translational Modifications

General Information of Drug Transporter (DT)
DT ID	DTD0014 Transporter Info
Gene Name	ABCB11
Transporter Name	Bile salt export pump
Gene ID	8647
UniProt ID	O95342

Post-Translational Modification of This DT
Overview ofABCB11 Modification Sites with Functional and Structural Information
Sequence	M S D S V I L R S I K K F G E E N D G F E S D K S Y N N D K K S R L Q D E K K G D G V R V G F F Q L F R F S S S T D I W L M F V G S L C A F L H G I A Q P G V L L I F G T M T D V F I D Y D V E L Q E L Q I P G K A C V N N T I V W T N S S L N Q N M T N G T R C G L L N I E S E M I K F A S Y Y A G I A V A V L I T G Y I Q I C F W V I A A A R Q I Q K M R K F Y F R R I M R M E I G W F D C N S V G E L N T R F S D D I N K I N D A I A D Q M A L F I Q R M T S T I C G F L L G F F R G W K L T L V I I S V S P L I G I G A A T I G L S V S K F T D Y E L K A Y A K A G V V A D E V I S S M R T V A A F G G E K R E V E R Y E K N L V F A Q R W G I R K G I V M G F F T G F V W C L I F L C Y A L A F W Y G S T L V L D E G E Y T P G T L V Q I F L S V I V G A L N L G N A S P C L E A F A T G R A A A T S I F E T I D R K P I I D C M S E D G Y K L D R I K G E I E F H N V T F H Y P S R P E V K I L N D L N M V I K P G E M T A L V G P S G A G K S T A L Q L I Q R F Y D P C E G M V T V D G H D I R S L N I Q W L R D Q I G I V E Q E P V L F S T T I A E N I R Y G R E D A T M E D I V Q A A K E A N A Y N F I M D L P Q Q F D T L V G E G G G Q M S G G Q K Q R V A I A R A L I R N P K I L L L D M A T S A L D N E S E A M V Q E V L S K I Q H G H T I I S V A H R L S T V R A A D T I I G F E H G T A V E R G T H E E L L E R K G V Y F T L V T L Q S Q G N Q A L N E E D I K D A T E D D M L A R T F S R G S Y Q D S L R A S I R Q R S K S Q L S Y L V H E P P L A V V D H K S T Y E E D R K D K D I P V Q E E V E P A P V R R I L K F S A P E W P Y M L V G S V G A A V N G T V T P L Y A F L F S Q I L G T F S I P D K E E Q R S Q I N G V C L L F V A M G C V S L F T Q F L Q G Y A F A K S G E L L T K R L R K F G F R A M L G Q D I A W F D D L R N S P G A L T T R L A T D A S Q V Q G A A G S Q I G M I V N S F T N V T V A M I I A F S F S W K L S L V I L C F F P F L A L S G A T Q T R M L T G F A S R D K Q A L E M V G Q I T N E A L S N I R T V A G I G K E R R F I E A L E T E L E K P F K T A I Q K A N I Y G F C F A F A Q C I M F I A N S A S Y R Y G G Y L I S N E G L H F S Y V F R V I S A V V L S A T A L G R A F S Y T P S Y A K A K I S A A R F F Q L L D R Q P P I S V Y N T A G E K W D N F Q G K I D F V D C K F T Y P S R P D S Q V L N G L S V S I S P G Q T L A F V G S S G C G K S T S I Q L L E R F Y D P D Q G K V M I D G H D S K K V N V Q F L R S N I G I V S Q E P V L F A C S I M D N I K Y G D N T K E I P M E R V I A A A K Q A Q L H D F V M S L P E K Y E T N V G S Q G S Q L S R G E K Q R I A I A R A I V R D P K I L L L D E A T S A L D T E S E K T V Q V A L D K A R E G R T C I V I A H R L S T I Q N A D I I A V M A Q G V V I E K G T H E E L M A Q K G A Y Y K L V T T G S P I S
PTM type	X-Acetylation X-N-glycosylation X-Oxidation X-Phosphorylation X-Phosphorylation X-Ubiquitination X-Ubiquitination X: Amino Acid

Acetylation
Lysine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	.			[1]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	436
Experimental Method	Co-Immunoprecipitation
Detailed Description	Acetylation at ABCB11 Lysine 436 has the potential to affect its expression or activity.
N-glycosylation
Asparagine	4 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Decreasing the activity of ABCB11			[2]
Role of PTM	Trafficking to Plasma Membrane
Affected Drug/Substrate	Taurocholate	Results for Drug	Decreasing the transport of taurocholate
Modified Residue	Asparagine	Modified Location	109
Modified State	Asparagine to Glutamine mutation
Experimental Material(s)	Madin-darby canine kidney II (MDCKII) cells
Experimental Method	Co-Immunoprecipitation
Detailed Description	Removal of the Glycosylation at ABCB11 Asparagine 109 (i.e. Asparagine to Glutamine mutation) have been reported to decrease its transport activity.
PTM Phenomenon2	Decreasing the activity of ABCB11			[2]
Role of PTM	Trafficking to Plasma Membrane
Affected Drug/Substrate	Taurocholate	Results for Drug	Decreasing the transport of taurocholate
Modified Residue	Asparagine	Modified Location	116
Modified State	Asparagine to Glutamine mutation
Experimental Material(s)	Madin-darby canine kidney II (MDCKII) cells
Experimental Method	Co-Immunoprecipitation
Detailed Description	Removal of the Glycosylation at ABCB11 Asparagine 116 (i.e. Asparagine to Glutamine mutation) have been reported to decrease its transport activity.
PTM Phenomenon3	Decreasing the activity of ABCB11			[2]
Role of PTM	Trafficking to Plasma Membrane
Affected Drug/Substrate	Taurocholate	Results for Drug	Decreasing the transport of taurocholate
Modified Residue	Asparagine	Modified Location	122
Modified State	Asparagine to Glutamine mutation
Experimental Material(s)	Madin-darby canine kidney II (MDCKII) cells
Experimental Method	Co-Immunoprecipitation
Detailed Description	Removal of the Glycosylation at ABCB11 Asparagine 122 (i.e. Asparagine to Glutamine mutation) have been reported to decrease its transport activity.
PTM Phenomenon4	Decreasing the activity of ABCB11			[2]
Role of PTM	Trafficking to Plasma Membrane
Affected Drug/Substrate	Taurocholate	Results for Drug	Decreasing the transport of taurocholate
Modified Residue	Asparagine	Modified Location	125
Modified State	Asparagine to Glutamine mutation
Experimental Material(s)	Madin-darby canine kidney II (MDCKII) cells
Experimental Method	Co-Immunoprecipitation
Detailed Description	Removal of the Glycosylation at ABCB11 Asparagine 125 (i.e. Asparagine to Glutamine mutation) have been reported to decrease its transport activity.
Oxidation
Cystine	2 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence ABCB11			[3]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	1117
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at ABCB11 Cystine 1117 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence ABCB11			[3]
Role of PTM	Potential impacts
Modified Residue	Cystine	Modified Location	1270
Experimental Method	Co-Immunoprecipitation
Detailed Description	Oxidation at ABCB11 Cystine 1270 has the potential to affect its expression or activity.
Phosphorylation
Serine	19 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence ABCB11			[4]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	2
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 2 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence ABCB11			[5]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	262
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 262 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence ABCB11			[6]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	286
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 286 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence ABCB11			[6]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	287
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 287 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence ABCB11			[7], [8]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	587
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 587 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence ABCB11			[9]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	593
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 593 has the potential to affect its expression or activity.
PTM Phenomenon7	Have the potential to influence ABCB11			[9], [10]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	683
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 683 has the potential to affect its expression or activity.
PTM Phenomenon8	Have the potential to influence ABCB11			[11], [12]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	686
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 686 has the potential to affect its expression or activity.
PTM Phenomenon9	Have the potential to influence ABCB11			[9], [13]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	690
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 690 has the potential to affect its expression or activity.
PTM Phenomenon10	Have the potential to influence ABCB11			[9], [13]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	694
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 694 has the potential to affect its expression or activity.
PTM Phenomenon11	.			[1]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	699
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 699 has the potential to affect its expression or activity.
PTM Phenomenon12	Have the potential to influence ABCB11			[9]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	701
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 701 has the potential to affect its expression or activity.
PTM Phenomenon13	Have the potential to influence ABCB11			[7], [14]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	704
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 704 has the potential to affect its expression or activity.
PTM Phenomenon14	Have the potential to influence ABCB11			[15]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	999
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 999 has the potential to affect its expression or activity.
PTM Phenomenon15	Have the potential to influence ABCB11			[16]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	1062
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 1062 has the potential to affect its expression or activity.
PTM Phenomenon16	Have the potential to influence ABCB11			[17]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	1114
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 1114 has the potential to affect its expression or activity.
PTM Phenomenon17	Have the potential to influence ABCB11			[17]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	1122
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 1122 has the potential to affect its expression or activity.
PTM Phenomenon18	Have the potential to influence ABCB11			[7], [9]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	1214
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 1214 has the potential to affect its expression or activity.
PTM Phenomenon19	Have the potential to influence ABCB11			[9]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	1217
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Serine 1217 has the potential to affect its expression or activity.
Threonine	6 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence ABCB11			[7], [9]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	586
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Threonine 586 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence ABCB11			[9]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	681
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Threonine 681 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence ABCB11			[18]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	858
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Threonine 858 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence ABCB11			[18]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	859
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Threonine 859 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence ABCB11			[16]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	1066
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Threonine 1066 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence ABCB11			[17]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	1121
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Threonine 1121 has the potential to affect its expression or activity.
Tyrosine	4 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence ABCB11			[9]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	687
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Tyrosine 687 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence ABCB11			[9]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	705
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Tyrosine 705 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence ABCB11			[5]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	1016
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Tyrosine 1016 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence ABCB11			[16]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	1064
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at ABCB11 Tyrosine 1064 has the potential to affect its expression or activity.
Ubiquitination
Lysine	5 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	.			[1]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	700
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at ABCB11 Lysine 700 has the potential to affect its expression or activity.
PTM Phenomenon2	.			[1]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	930
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at ABCB11 Lysine 930 has the potential to affect its expression or activity.
PTM Phenomenon3	.			[1]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	930
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at ABCB11 Lysine 930 has the potential to affect its expression or activity.
PTM Phenomenon4	.			[1]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	1193
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at ABCB11 Lysine 1193 has the potential to affect its expression or activity.
PTM Phenomenon5	.			[1]
Role of PTM	Potential impacts
Modified Residue	Lysine	Modified Location	1263
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at ABCB11 Lysine 1263 has the potential to affect its expression or activity.
Unclear Residue	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Affecting the surface membrane expression of ABCB11			[19]
Role of PTM	Degradation via Proteosome
Experimental Material(s)	Madin-darby canine kidney II (MDCKII) cells
Experimental Method	Co-Immunoprecipitation
Detailed Description	Ubiquitination at ABCB11 have been reported to affect its surface membrane expression.

References
1	15 years of PhosphoSitePlus?: integrating post-translationally modified sites, disease variants and isoforms. Nucleic Acids Res. 2019;47(D1):D433-D441.
2	Two N-linked glycans are required to maintain the transport activity of the bile salt export pump (ABCB11) in MDCK II cells. Am J Physiol Gastrointest Liver Physiol. 2007 Mar;292(3):G818-28.
3	A Quantitative Tissue-Specific Landscape of Protein Redox Regulation during Aging. Cell. 2020 Mar 5;180(5):968-983.e24.
4	Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics. 2012 Sep;11(9):651-68.
5	Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics. 2014 Jul;13(7):1690-704.
6	Large-Scale Reanalysis of Publicly Available HeLa Cell Proteomics Data in the Context of the Human Proteome Project. J Proteome Res. 2018 Dec 7;17(12):4160-4170.
7	UniProt: a worldwide hub of protein knowledge. Nucleic Acids Res. 2019 Jan 8;47(D1):D506-D515.
8	A fast sample processing strategy for large-scale profiling of human urine phosphoproteome by mass spectrometry. Talanta. 2018 Aug 1;185:166-173.
9	An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62.
10	Competitive regulation of modular allosteric aptazymes by a small molecule and oligonucleotide effector. RNA. 2005 Oct;11(10):1514-20.
11	Comparison of the Pharmacokinetics and Pharmacodynamics of Single-Dose Tenofovir Vaginal Film and Gel Formulation (FAME 05). J Acquir Immune Defic Syndr. 2018 Feb 1;77(2):175-182.
12	Phosphoproteome Integration Reveals Patient-Specific Networks in Prostate Cancer. Cell. 2016 Aug 11;166(4):1041-1054.
13	Large-scale proteome quantification of hepatocellular carcinoma tissues by a three-dimensional liquid chromatography strategy integrated with sample preparation. J Proteome Res. 2014 Aug 1;13(8):3645-54.
14	Non-alcoholic fatty liver disease phosphoproteomics: A functional piece of the precision puzzle. Hepatol Res. 2017 Dec;47(13):1469-1483.
15	The calcium-dependent protein kinase 3 of toxoplasma influences basal calcium levels and functions beyond egress as revealed by quantitative phosphoproteome analysis. PLoS Pathog. 2014 Jun 19;10(6):e1004197.
16	An integrated strategy for highly sensitive phosphoproteome analysis from low micrograms of protein samples. Analyst. 2018 Jul 23;143(15):3693-3701.
17	iTRAQ labeling is superior to mTRAQ for quantitative global proteomics and phosphoproteomics. Mol Cell Proteomics. 2012 Jun;11(6):M111.014423.
18	dbPTM in 2022: an updated database for exploring regulatory networks and functional associations of protein post-translational modifications. Nucleic Acids Res. 2022 Jan 7;50(D1):D471-D479. (ID: ABCBB_HUMAN)
19	Short-chain ubiquitination is associated with the degradation rate of a cell-surface-resident bile salt export pump (BSEP/ABCB11). Mol Pharmacol. 2009 Jan;75(1):143-50.

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Detail Information of Post-Translational Modifications

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