Detail Information of Post-Translational Modifications

General Information of Drug Transporter (DT)
DT ID	DTD0010 Transporter Info
Gene Name	SLC22A1
Transporter Name	Organic cation transporter 1
Gene ID	6580
UniProt ID	O15245

Post-Translational Modification of This DT
Overview ofSLC22A1 Modification Sites with Functional and Structural Information
Sequence	M P T V D D I L E Q V G E S G W F Q K Q A F L I L C L L S A A F A P I C V G I V F L G F T P D H H C Q S P G V A E L S Q R C G W S P A E E L N Y T V P G L G P A G E A F L G Q C R R Y E V D W N Q S A L S C V D P L A S L A T N R S H L P L G P C Q D G W V Y D T P G S S I V T E F N L V C A D S W K L D L F Q S C L N A G F L F G S L G V G Y F A D R F G R K L C L L G T V L V N A V S G V L M A F S P N Y M S M L L F R L L Q G L V S K G N W M A G Y T L I T E F V G S G S R R T V A I M Y Q M A F T V G L V A L T G L A Y A L P H W R W L Q L A V S L P T F L F L L Y Y W C V P E S P R W L L S Q K R N T E A I K I M D H I A Q K N G K L P P A D L K M L S L E E D V T E K L S P S F A D L F R T P R L R K R T F I L M Y L W F T D S V L Y Q G L I L H M G A T S G N L Y L D F L Y S A L V E I P G A F I A L I T I D R V G R I Y P M A M S N L L A G A A C L V M I F I S P D L H W L N I I I M C V G R M G I T I A I Q M I C L V N A E L Y P T F V R N L G V M V C S S L C D I G G I I T P F I V F R L R E V W Q A L P L I L F A V L G L L A A G V T L L L P E T K G V A L P E T M K D A E N L G R K A K P K E N T I Y L K V Q T S E P S G T
PTM type	X-N-glycosylation X-Phosphorylation X: Amino Acid

N-glycosylation
Asparagine	1 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC22A1			[1]
Role of PTM	Potential impacts
Modified Residue	Asparagine	Modified Location	71
Experimental Method	Co-Immunoprecipitation
Detailed Description	N-linked Glycosylation at SLC22A1 Asparagine 71 has the potential to affect its expression or activity.
Phosphorylation
Serine	7 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC22A1			[2]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	213
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Serine 213 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC22A1			[3]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	230
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Serine 230 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC22A1			[3]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	232
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Serine 232 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC22A1			[4]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	331
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Serine 331 has the potential to affect its expression or activity.
PTM Phenomenon5	Have the potential to influence SLC22A1			[4]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	333
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Serine 333 has the potential to affect its expression or activity.
PTM Phenomenon6	Have the potential to influence SLC22A1			[5]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	549
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Serine 549 has the potential to affect its expression or activity.
PTM Phenomenon7	Have the potential to influence SLC22A1			[5]
Role of PTM	Potential impacts
Modified Residue	Serine	Modified Location	552
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Serine 552 has the potential to affect its expression or activity.
Threonine	4 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Have the potential to influence SLC22A1			[3]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	222
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Threonine 222 has the potential to affect its expression or activity.
PTM Phenomenon2	Have the potential to influence SLC22A1			[3]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	225
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Threonine 225 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC22A1			[6]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	510
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Threonine 510 has the potential to affect its expression or activity.
PTM Phenomenon4	Have the potential to influence SLC22A1			[5], [7]
Role of PTM	Potential impacts
Modified Residue	Threonine	Modified Location	541
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Threonine 541 has the potential to affect its expression or activity.
Tyrosine	3 PTM Phenomena Related to This Residue		Click to Show/Hide the Full List
PTM Phenomenon1	Potentially affecting the transporter			[8], [9]
Role of PTM	On/Off Switch
Modified Residue	Tyrosine
Modified State	Tyrosine kinase inhibitors (Pazopanib)
Experimental Method	Co-Immunoprecipitation
Detailed Description	Removal of the Phosphorylation at SLC22A1 Tyrosine (i.e. Tyrosine kinase inhibitors (Pazopanib)) have been reported to have the potential to affect the transporter.
PTM Phenomenon2	Have the potential to influence SLC22A1			[3]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	221
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Tyrosine 221 has the potential to affect its expression or activity.
PTM Phenomenon3	Have the potential to influence SLC22A1			[5]
Role of PTM	Potential impacts
Modified Residue	Tyrosine	Modified Location	543
Experimental Method	Co-Immunoprecipitation
Detailed Description	Phosphorylation at SLC22A1 Tyrosine 543 has the potential to affect its expression or activity.

References
1	dbPTM in 2022: an updated database for exploring regulatory networks and functional associations of protein post-translational modifications. Nucleic Acids Res. 2022 Jan 7;50(D1):D471-D479. (ID: S22A1_HUMAN)
2	An integrated strategy for highly sensitive phosphoproteome analysis from low micrograms of protein samples. Analyst. 2018 Jul 23;143(15):3693-3701.
3	Citric acid-assisted two-step enrichment with TiO2 enhances the separation of multi- and monophosphorylated peptides and increases phosphoprotein profiling. J Proteome Res. 2013 Jun 7;12(6):2467-76.
4	Phosphoproteome Analysis Reveals Differential Mode of Action of Sorafenib in Wildtype and Mutated FLT3 Acute Myeloid Leukemia (AML) Cells. Mol Cell Proteomics. 2017 Jul;16(7):1365-1376.
5	FAIMS and Phosphoproteomics of Fibroblast Growth Factor Signaling: Enhanced Identification of Multiply Phosphorylated Peptides. J Proteome Res. 2015 Dec 4;14(12):5077-87.
6	iTRAQ labeling is superior to mTRAQ for quantitative global proteomics and phosphoproteomics. Mol Cell Proteomics. 2012 Jun;11(6):M111.014423.
7	UniProt: a worldwide hub of protein knowledge. Nucleic Acids Res. 2019 Jan 8;47(D1):D506-D515.
8	Inhibition of OCT2, MATE1 and MATE2-K as a possible mechanism of drug interaction between pazopanib and cisplatin. Pharmacol Res. 2016 Aug;110:89-95.
9	A phosphotyrosine switch regulates organic cation transporters. Nat Commun. 2016 Mar 16;7:10880.

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Detail Information of Post-Translational Modifications

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